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After 5 min of pre-incubation at 37 C, 10 L of 20 mM nicotinamide adenine dinucleotide phosphate (NADPH) tetrasodium salt in PBS was added as cofactor to initiate the reaction, followed by incubation for a further 60 min at a shaking frequency of 550 rpm

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References 1 FASEB JournalBiological activities of thymosin beta-4 mapped to active peptide sequences, including the fragment marketed as TB-500

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