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For shRNA expression, the culture medium was supplemented daily with 5 mM doxycycline

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In UK clinical settings, the recognised standard diluent for injectable medicines is Water for Injections BP (single-use, preservative-free), and the choice of diluent must always follow the licensed product's Summary of Product Characteristics (SmPC)

1988;27 Suppl 1:86-93

Short-chain fatty acids are microbial fermentation products exert neuroprotective effects via epigenetic regulation, including inhibition of histone deacetylases and promotion of neurotrophic signaling [627, 628]