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Vitamin C supplementation for prevention and treatment of pneumonia

Subsequently, the medium was replaced with OPTI-MEM supplemented with 5% FBS and 1% sodium pyruvate

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doi: 10.1002/jcp.24839

Using a genome-wide CRISPR screen, mitochondrial ferredoxin (FDX1), and lipoyl synthase (LIAS) were identified as key regulators of Cu toxicity, 2,257 and genetic knockout of either FDX1 or LIAS leads to an accumulation of pyruvate and -ketoglutarate, reducing protein lipoylation and inhibiting Cu-induced cell death (Fig