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The supernatant containing His-tagged histones was then incubated with NiNTA resin (Complete His-Tag Purification Resin, Roche) equilibrated with buffer B (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 6 M urea, 5 mM imidazole, and 5% glycerol) for 1 h with end-over-end mixing at 4 C

In addition, although our mechanistic gain- and loss-of-function studies were supported by extensive in vivo validation, part of the functional characterization was performed using HL60-derived neutrophil cells

Sullivan A, Nord CE, Evengrd B

[DOI] [PubMed] [Google Scholar] 248.Martinez-Galisteo E, Padilla CA, Holmgren A, Barcena JA