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We used 15 N-labeled FOXO4 FHD and p53 TAD with S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSL)-labeled Cys46 for the PRE experiments

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For best results, use consistently for at least 3 months.

For calcium channel pharmacology studies, pre-treat neurons for 30 minutes with 10 M nifedipine prior to this compound treatment