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The column was washed with 15 column volumes of PPS buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4) at a flow rate of 5 ml min 1

35 HaapakoskiR.EbmeierK

Nakashima, H

Thereby, standardizing the source and extraction and purification procedure is one primary and critical step for polysaccharide toward enhancing their medicinal value