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The CRISPR sgRNA was then microinjected into fertilised oocytes obtained from C57Bl/6 homozygous double humanised FcRn/albumin mouse line (genOway proprietary model 27 )

As historian Benjamin Fortna has detailed, in the early 20th century it was still common to see Ottoman maps that included Tunisia (a French protectorate since 1881), Egypt (largely independent since 1805, occupied by Britain since 1882), Bulgaria (autonomous since 1878), Cyprus (a British protectorate since 1878), and even Somalia (which had only been Ottoman in a nominal sense to begin with)

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Thus, in consistent with the CC1 immuno-staining, these ISH results also clearly demonstrated a quite rapid response, i.e

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