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As shown in Figure S8, we first treated HCECs seeded on coverslip A with FITC-labeled CeNPs for 24 h, and then this coverslip was co-cultured with a second coverslip (coverslip B) in fresh culture medium for another 24 h

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Finally, IGF-1R signaling may be modulated by processes such as acetylation (72), glycosylation (73), prenylation (74), neddylation (75), SUMOylation (76) or through interactions with a specific cell membrane inhibitor named inceptor (77)

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Extended storage of reconstituted solution: For storage beyond 30 days, prepare sterile aliquots and freeze at 20 C (4 F)

Then, the effect of metabolic stress generated by HFD on the circadian clock of MGs and its possible underlying mechanisms were explored by bioinformatics analysis and the detection of diurnal oscillations of lipid droplets in MGs