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A new study by scientists at Osaka Metropolitan University investigated the changes in polysulfide biosynthesis in broccoli during germination

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Journal of Clinical Endocrinology and Metabolism , 1990

albicans (SC5314 and 101) overnight cultures were seeded in 200 L RPMI-1640 for Seahorse (Agilent Technologies, Santa Clara, CA), supplemented with 2 mM l-Glutamine, 1 mM sodium pyruvate and 11 mM d-Glucose with or without 10 mg/mL albumin (Sanquin Plasma Products B.V.) as 10 4 yeast cells/well in a Seahorse 96-well cell culture plate