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Optimization and technical considerations for the dye-exclusion protocol used to assess blood-brain barrier integrity in adult drosophila melanogaster

The ancient Egyptian civilization used black cumin as a preservative in the process of mummification [16] probably due to its antibacterial and insect repellent actions [17,18]

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To maintain fluid balance, all of the mice were supplemented with 1 mL of prewarmed PBS administered intraperitoneally directly after surgery

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Rinse it off and repeat the procedure for a few more days