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A solution containing 1.75 mg/ml fibrinogen and 0.9 g/mL FXIIIa in 50 mM Tris HCl buffer of pH 7.4 containing 10 mM CaCl 2 was incubated with different concentrations of EA (5 5000 M), and then clotted in the presence of human -thrombin (1.25 g/mL)

however, three GPX4-independent pathways have been determined

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TrxR transfers electrons from NADPH to reduce oxidized thioredoxin (Trx-S 2 ) to Trx-(SH) 2 , which provides reducing equivalents in the enzymatic removal of H 2 O 2 and other organic hydroperoxides by Prx [33]

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Ann Med 53(1):17271736