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In this trial, 342 men with sildenafil-failure ED were given either intranasal PT-141 (10 mg dose) or placebo on demand over ~48 weeks.The outcomes demonstrated a clear benefit of bremelanotide: About 33.5% of men on bremelanotide achieved a positive clinical response (improved erections sufficient for intercourse) compared to only 8.5% on placebo

mRNA levels of potential c-Jun target genes were analysed by real-time PCR

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S1P is a signaling sphingolipid produced by the phosphorylation of sphingosine by two sphingosine kinases, SphK1 and SphK2 (Maceyka et al., 2012)

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