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Relative band pixel intensities were semiquantitatively analyzed using ImageJ software (version 1.8.0, National Institutes of Health, Bethesda, MD, United States), and protein loading was normalized using an antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-32233, Santa Cruz Biotechnology, Santa Cruz, CA)

Before you see your primary care doctor, physical therapist, chiropractor, or other provider, it is important that you have a thorough history and physical examination by an orthopedic surgeon to establish the correct diagnosis