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Arch Biochem Biophys (2025) 768:110366

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Results and discussion Method optimization and development HPTLC method Experiments employing various solvent systems, such as ethyl acetate + cloroform (4:4, 8:2, 9:1, 2:8, and 9.5:0.5, v/v), revealed inadequate separation of the compounds under investigation and the appearance of tailed peaks