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Standard laboratory practice limits freeze-thaw cycles to a maximum of three before the vial is considered compromised

This is done by stimulating the expression of two vitally important proteins, arylalkylamine-N-acetyltransferase (AANAT) and the transcriptional protein pCREB

If a researcher cannot train consistently because of injury or poor recovery, the downstream muscle stimulus is limited

Nature 413:118121 Lipton AJ, Johnson MA, Macdonald T, Lieberman NW, Gozal D, Gaston B (2001) S-nitrosylothiols signal ventilatory response to hypoxia

doi:10.1038/s41591-022-01978-x

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