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Glutamine synthetase (GS) can maintain the phenotype of M2 macrophages, and the inhibition of GS skews M2-polarized macrophages toward the M1-like phenotype [27]

Farrand et al

Ferdous Z, Elzaki O, Beegam S, Zaaba NE, Tariq S, Adeghate E, et al

For the assessment of DNA damage, surface-stained cells were fixed with 4% ultrapure paraformaldehyde (Polysciences) for 15 min, permeabilized with ice-cold 100% methanol (Supelco) for 20 min, and subsequently stained for -H2AX (phopho-Ser139) for 1 h