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DNA extraction and c-Myc copy number variation by droplet digital PCR After drug treatment, tumour cells grown as monolayers were treated with Trypsin, pelleted and washed once with PBS before being stored at -80 C

doi: 10.1016/j.ijgo.2007.10.024 32 CriderKSYangTPBerryRJBaileyLB

This LASSS-HGF strategy could be adaptable to aging 32,41,42,68,69,70 and several disorders and diseases that show the generation and accumulation of nitrated HGF and particular nitrated proteins at specific sites, including cardiovascular damage 71,72 , chronic obstructive pulmonary disease (COPD) 73 , colon and lung cancer 74,75,76 , Alzheimers and Parkinsons diseases 44 46 ,77,78

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