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World Mycotoxin J

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The samples were then diluted with 0.1 M Tris-HCl (pH 7.5) at a final immobilization volume of 1 ml and incubated in a rotary shaker for 1 h at 4 C

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In erythrocytes, 2,3-BPG also acts as an allosteric inhibitor of hemoglobin, lowering its affinity for oxygen