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Cells were maintained in 5% CO 2 at 37 C and grown in DMEM medium containing 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, penicillin (50 U/mL) and streptomycin (100 mg/mL) (All medium and supplements were obtained from GIBCO)

Eligible patients with AUDIT-C 3 (n=30) proceeded to structured MINI interview and TLFB

It has also been found that glutamine can participate in the metabolism of cancer stem cells (CSCs) [29]

Frequency: Six times per week Study Duration: According to data, L-glutathione may be administered continuously for four to eight weeks

doi: 10.1016/j.cell.2011.06.034 28

Dr Raghu reports receiving fees for serving as an ad hoc discussant from Bristol Myers Squibb and United Therapeutics, receiving consulting fees from Veracyte, receiving fees for reviewing investigator initiated research grant proposals from Boehringer Ingelheim, providing unpaid consulting services for Biogen, Bellerophon Therapeutics, Blade Therapeutics, FibroGen, Nitto, Roche/Genentech, and Novartis, serving (unpaid) on a data and safety monitoring board for Avalyn, and No other potential conflict of interest relevant to this manuscript