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The importance of S87 for BCL-2 stability and activity was further demonstrated as mutation of S87A drives degradation of the protein (De Chiara et al., 2006)

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For PCR analysis, 2 L of the lysate was used as a template with the following primers: F1: 5-GCCAGCTACTGCATATATTGAGACC-3 R1: 5-GGATATAGAGAAGCCCTAGAACAGGG-3 R2: 5-CATGTCGTGTTGGTCAAATAAGCAC-3 PCR amplification using primers F1/R1 generated a 278 bp product for wild-type (WT) alleles and a 382 bp product for floxed alleles

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The blend supports natural sleep and stress regulation systems rather than forcing pharmacological suppression

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