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Methods Cell culture HepG2 cells were cultured in DMEM (Gibco), and supplemented with 10% dialysed fetal bovine serum (FBS), 5% non-essential amino acids, 1% penicillin/streptomycin, 5.6 mM glucose, and 2 mM l-glutamine

The review also recapitulates the disruption of the tripartite synapse with a focus on perturbations of the homeostatic astrocytic function as a key driver to modulate the molecular and physiological processes toward neurodegenerative diseases

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