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THP-1 cells are usually treated with phorbol 12-myristate 13-acetate (PMA) in order to differentiate them into macrophages (Riendeau and Kornfeld, 2003)

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Two possible scenarios were considered to explain the distinct appearance of cyanobacterial nozzles: either the physiological structures are monomeric rings, and the observed dimers formed during isolation, or the nozzles function as dimers, indicating a plasticity in certain secretins to adopt novel structural arrangements