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thereafter, 50 ml of precultures were induced with 450 ml of expression medium (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 bacto peptone (Gibco, 211677), 20 g l 1 d-galactose (Panreac, A1131), 67 mM potassium phosphate monobasic buffer pH 6.0 (Thermo Scientific, 424200250), 25 g l 1 ethanol (Scharlau, ET00062500), 2 mM copper sulfate (Merck, C7631) and 25 g ml 1 chloramphenicol (Merck, C0378)) in a 2.5-l baffled flask (Quimigen, 931136-B) (OD 600 = 0.1)

26 , e3608 (2024)

Lih-Brody L, Powell SR, Collier KP, Reddy GM, Kahn E, Weissman GS, Katz S, Floyd RA, McKinley MJ, Fisher SE, and Mullin GE (1996)

Adoptive transfer As described 52 , splenocytes were collected by gently pushing spleens through a 100-m strainer, and red blood cells were lysed with ACK lysing buffer (Gibco, A10492-01)

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