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doi: 10.2967/jnumed.112.111112 200 WangJ.HeX.MengH.LiY.DmitrievP.TianF.et al

SH-SY5Y and BV-2 cells were grown in 6-well plates at densities of 1 106 and 5 105 cells/well, respectively

The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock