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Biochem J 2006

The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system

Importantly, the position of RICTOR within mTORC2 explains the insensitivity of the complex during acute treatment with rapamycin because the FRB domain of mTOR in mTORC2 is sterically hindered by RICTOR, preventing the FKBP12rapamycin complex from binding

The light energy heats the follicle and disrupts its ability to produce new hair

Studies by Coolican et al