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Required materials: BPC 157 lyophilized vial (typically 5 mg or 10 mg) Bacteriostatic water (BAC water, 0.9% benzyl alcohol) for injection U-100 insulin syringes (1 mL capacity, 2831 gauge) Alcohol swabs (70% isopropyl) Sterile nitrile gloves A clean, flat workspace (ideally wiped with 70% isopropyl alcohol) Sharps disposal container Workspace and vial preparation: Warming the lyophilized vial to room temperature for 1020 minutes before opening reduces condensation on the stopper

UFA levels in CSF were quantified using stable isotope dilution GC/MS as previously described (Fonteh et al., 2014)

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doi: 10.1016/j.advenzreg.2006.01.007 250 MaSZhouXWangYLiZWangYShiJet al

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A standard curve prepared with the supplied ATP standard was used for quantification and ATP concentrations were normalized to total cellular protein content (mol ATP/mg protein)