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Next, livers were harvested, and liver cells were disassociated by digestion with Pronase/collagenase solution and filtered through a nylon filter (Corning) to remove undigested tissues and debris

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Low CuCl 2 concentrations (0.2-0.2.4mmol/l) did not induce significant differences, while higher concentrations (0.61.6.0mmol/l) led to significant toxicity in HepG2 ATP7B-KO cells (Fig