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Anxiety can deepen the same loop, since excessive worry often brings muscle tension, restlessness, and trouble concentrating, symptoms detailed by the Mayo Clinic in its guide to generalized anxiety disorder

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The immunostained sections were scanned on a NanoZoomer NDP system (Hamamatsu Photonics, Shizuoka, Japan) with 20 resolution, or on a FV3000 confocal microscope (Olympus, Tokyo, Japan) with 4, 10 or 20 resolution

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