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Komaki R, Wei X, Allen P, Roth JA, Swisher S, Rice DC , Walsh GL, Fossella FV, Erasmus JJ, Cox JD

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Recombinant N-terminally MRGS(H) 6 -tagged wild-type and mutant ScGrx7 and HsGrx5 C122S (without their N-terminal targeting sequences 19 ) were purified after lysozyme treatment and sonication by Ni-NTA affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl, and 50 mM sodium phosphate, pH 8.0 11,19,25,42

The enhanced activity of antioxidant enzymes observed under PV shading, despite the lower accumulation of oxidative stress markers, may reflect a physiological priming or acclimation response

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