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Cell viability was measured using the CellTiterBlue reagent (Promega, United States) according to the manufacturers protocol with Fluoroskan Ascent FL Microplate Reader (Thermo Labsystems, Waltham, MA, United States)

Knockout of Cyp26a1 and Cyp26b1 during postnatal life causes reduced lifespan, dermatitis, splenomegaly, and systemic inflammation in mice

Anticancer Res 2012;32:2835-41

Jan-Mar 2017;6(1):115-119

The occurrence of leukemia may cause disorders in the metabolism of trace elements, resulting in an imbalance between the oxidative and antioxidant systems and accelerating the development of the disease