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We starved ES cells or Arg or Leu in combination with inhibitors of vital metabolic checkpoints including GSH synthesis, NADPH production, and 1-carbon metabolism (Gclc, Cth, G6pdx, Mthfd2, or Shmt2 using L-buthionine-sulfoximine (BSO), DL-propargylglycine (PAG), 6-aminonicotinamide (6AN), DS18561882 (DS), or SHIN1, respectively)

doi: 10.1186/s12931-024-02969-x 143 ZhangMWangJLiuRWangQQinSChenYet al

PMID: 10891638 Claims .,,

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Tendril anatomy: A tool for correct identification among Cucurbitaceous taxa

These findings strongly indicate that the cells had depleted all available choline in the media, and intracellular concentrations fell below the optimal level