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After dissociation and lysis, cells were washed in FACS buffer once and resuspended in 5 ml FACS buffer

RNA isolation and RT-qPCR Total RNA was extracted from pooled retinal tissue from 2 eyes of a single adult fish (individual fish served as a biological replicate) using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD) according to the manufacturers protocol for animal tissue

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Stress related shift toward inflammaging in cosmonauts after long-duration space flight

Curr Opin Anaesthesiol

Breaking this cycle at any point can theoretically resolve a flare, but sustainable improvement requires addressing the cycle at multiple points simultaneously, or finding an intervention that modulates the central controlling mechanisms like NF-kB