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What is known so far is that cytokines-mediated neuroinflammation leads to dysregulations in the HPA axis, where the central regulator is cortisol releasing factor (CRF)

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GST tagged fusion proteins bind to glutathione agarose (column 2) and, after washing, are eluted with elution buffer with excess glutathione (column 3)

The linear gradient profile was as follows: 0 min, 100% B

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