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The cell pellet was incubated with Annexin V-FITC and propidium iodide staining solution for 1020 min at room temperature (2025 C) in the dark and then placed in an ice bath

3 Thus, in general, ferroptosis is regulated mostly by lipid metabolism, iron homeostasis, and redox system homeostasis

Lin Z, Huang L, Li SL, Gu J, Cui X, Zhou Y

Nature 532 , 195200 (2016)

Increased Oil-red O and Cd68 staining were also evident in the aortic roots of EC- Slc7a11 -/- mice, thereby reinforcing that Slc7a11 ablation enhanced the atherosclerosis susceptibility (Figure 5E)