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The loss of affinity can be mitigated by optimizing the type and length of linker, but the development of site-specific multivalent nanobodies construction methods has a wide range of applications

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We also examined the effects of BME on MDCK and HEK293T cells

The bifunctional complex according to claim 1, wherein a linker-oligonucleotide tag attachment point is separated from the molecule by from 5 to 50 atomic bonds

doi: 10.1016/j.cyto.2011.04.001 49

These cells exhibit a profound resistance to current treatment modalities, including radiotherapy and chemotherapy, largely attributable to their enhanced DNA repair capabilities, representing a key obstacle to the successful treatment of glioma and a significant factor in treatment failure and disease recurrence