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Significant shifts in microbiota family composition caused by the antimicrobial in both groups that included decreases in the proportion of Lachnospiraceae , Coriobacteriaceae and unidentified Clostridiales

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decreased serotonin synthesis in the dorsal thalamus, hippocampus, lateral geniculate body and hypothalamu

Muscle crush injury Right hind limbs were shaved, and using special system a force of 0.727 Ns/cm2 was delivered to a maximum diameter of gastrocnemius muscle complex 2 cm proximal to the insertion of the Achilles tendon (12)

then add 50 L Tween-80 and mix evenly