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doi: 10.1177/03009858211012949

for instance, a mouse model of Parkinsons disease showed significantly reduced hm 5 C levels in the hippocampus, substantia nigra, and striatum [182] However, a significant caveat to these findings is that the analysis was performed on the total RNA pool rather than the purified mRNA fraction, which precludes definitive conclusions regarding the specific role of hm 5 C within the messenger RNA landscape

This is one reason why researchers interested in microdosing often reconstitute with more water (3mL or even 4mL) to get a lower concentration that makes small doses easier to measure

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