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Mechanistic studies have linked celastrols protective effects to pathways such as the reduction in HO-1-mediated autophagy and the SIRT1-mediated inhibition of the EZH2-related Wnt/-catenin pathway [22, 39]

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The sections were then washed with PBS and treated with secondary antibody (diluted in PBS containing 0.3% Triton X-100) for 2 h at room temperature

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