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Step 2: Transfer the solution to the included empty 10 ml vial

And use the calculator to plan a complete protocol timeline that allows for meaningful data collection at each dose level

Small changes make a big difference

Feeling unwell

The pain wakes me up at night

Groups at higher risk of deficiency include older adults (due to reduced stomach acid production), people following vegan or strict vegetarian diets (as B12 is naturally found almost exclusively in animal products), individuals with pernicious anaemia or other malabsorption conditions, those taking certain medications such as metformin or proton pump inhibitors long-term, and people with significant nitrous oxide exposure (including recreational use), which can inactivate B12 in the body