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DMEM was purchased from Nanjing BioChannel Biotechnology Co., Ltd

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The penetration of antimicrobials into a biofilm depends on the thickness of the biofilm, the reactivity and diffusion of the agent within the biofilm, the sorption of the biofilm, and the dose concentration of the agent (Stewart, 2015)

Vidal-Casariego A, Burgos-Pelez R, et al

Of the 20 amino acids the human body needs to function, nine are actually essential