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To isolate the barcode and IS, we allowed 3 mismatches in the 23 bp invariant sequence upstream and up to 6 mismatches in the 50 bp downstream of the barcode using a Hamming distance function to compare the expected and actual sequences

Participants were excluded if they were missing blood data or had depression at wave 1 or did not complete 4-year follow-up, including assessment of incident depression at both wave 2 and wave 3

This suggests that neuronal degeneration may lead to local release of iron, which is subsequently scavenged and stored by microglia to maintain brain iron homeostasis (346)

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