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Therefore, the subsequent PI3K/Akt impairment suppresses this vital Nrf2-mediated antioxidant defenses while concurrently activating NFB-driven inflammation, thereby exacerbating mitochondrial ROS production and cytokine release (e.g., IL-6, TNF- , IL-1) in diabetic complications (41, 44)

Isopropyl--D-thiogalactopyranoside (IPTG) was added to the final concentration of 0.4 mM to induce the expression of recombinant protein at 25C for at least 5 h

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doi: 10.1016/j.cmet.2015.09.010 323 LiXStanislausSAsuncionFNiuQ-TChinookoswongNVillasenorKet al

These efforts to advance protein stability by strategically inserting cross-linking bridges demonstrate the power of site-selective protein editing and the covalent stabilization approach to produce novel enzymes for various biochemical and biomedical applications