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The random and site-saturation libraries contained a similar number of mutations on average (random = 2.79, site-saturation = 2.99), therefore, the lower fold induction in the site-saturation library may be attributed to less conservative mutations accessible via the NNS codon compared with single-nucleotide substitutions (Supplementary Fig

Next, measure out 1/2 cup of room temperature water per gallon of paint, then pour it in slowly while stirring the paint in upward and downward spirals

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