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Description

After staining for cell surface antigens, the cells were fixed with fixation buffer (420801, Biolegend), permeabilized with permeabilization wash buffer (421002, Biolegend), and stained for intracellular antigens with anti-mouse APC FoxP-3 (320011, Biolegend), FITC Granzyme B (515403, Biolegend), and APC IFN (505809, Biolegend)

This research studied the cytotoxicity of these variables via in vitro keratinocyte and fibroblast cell tests

As reported in Figure 4B, we observed a concentration-dependent reduction in viral RNA synthesis with all four DENV serotypes, thus pointing out a potential pan-dengue antiviral activity for hit 3