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This animal study was performed in accordance with the following: - UK guidance on the operation of the Animals (Scientific Procedures) Act of 1986 and associated guidelines - EU Directive 2010/63 for the protection of animals used for scientific purposes - The NIH (National Research Council) Guide for the Care and Use of Laboratory Animals The study also complies with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines

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Expression from the human occludin promoter is affected by tumor necrosis factor alpha and interferon gamma

Ultrathin sections (700 thick) were obtained by means of an ultramicrotome (Reichert Ultracut E) equipped with a diamond knife

The ability of gut microbiota to communicate with the brain and in turn induce behavioral changes is emerging as an interesting concept in autism