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BPC-157 was named first

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it upregulates heme oxygenase-1 (HO-1) and other antioxidant and detoxification genes ( + by reducing the heme-associated ferric iron (Fe + ) to Fe + ( + must then be tightly regulated, either stored within ferritin or exported by FPN, to avoid intracellular accumulation and subsequent ferroptosis ( Hepcidin can induce the internalization and degradation of FPN, thereby reducing cellular iron export and elevating intracellular iron levels ( In addition to these canonical signaling pathways, recent single-cell transcriptomic studies have revealed marked heterogeneity in ferroptosis susceptibility among chondrocyte subpopulations in OA cartilage

After assessment of its purity and integrity via a Nanophotometer (Implen, Mnchen, Germany) and evaluation of its concentration, RNA was reverse transcribed into cDNA with GoScript Reverse Transcription Mix, Oligo(dT) (Promega)

Espinosa-Oliva et al., 2024)