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Golden Gate reaction mixtures were prepared as follows: 40 fmol of each DNA insert or plasmid, 1.5 l of T4 DNA ligase (400 U/l, NEB), 1 l of restriction enzyme, 2.5 l of 10 ligation buffer, and sterile water to bring the final volume to 25 l

There were no significant differences between the two cuprizone groups (p = 0.9017)

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The level of CYS in SI1 on d14 was higher in the HGSH group than in the CON and LGSH groups ( p = 0.007)