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[18] The glyoxalase system, consisting of glyoxalase I and II, uses glutathione to convert the reactive dicarbonyl methylglyoxal, a by-product of glycolysis, into Dlactate

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After centrifugation (10,000 g for 5 min at 4 C), supernatants were transferred to fresh tubes pre-chilled on ice, and the protein concentrations of supernatants were measured

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