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On day 1, PHMs were collected with subculture reagents (Lonza) and seeded onto collagen I-coated 96-well plates (Corning) at a density of 3200 cells/well

Pro-oxidant molecules are kept at low levels in the fibroblasts of the patient Because of the peculiar regulation undergone by cellular antioxidant defences, we decided to assess intracellular ROS levels (see Methods)

Ozkaya O, Mekin S, Hakan K

[148:47] Brigham Life's a trip

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doi: 10.1158/0008-5472.Can-09-2213 74 ShahYMMatsubaraTItoSYimSHGonzalezFJ