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RRID: AB_314449) in 0.1 ml incubation buffer for 30 min at 25C

RMSF and solvent-accessible surface area (SASA) (Tabrez et al., 2022)

demonstrated that Erastin treatment in fibroblasts and fibrosarcoma cells robustly induced the conversion of LC3I to LC3II and the formation of GFP-LC3 puncta, which are definitive hallmarks of autophagy activation (49)